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AI-powered inclusivity, exclusivity, variant impact, and multiplex cross-hybridization for PCR and NGS assays — scored, explained, and documented in under three minutes.
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BLASTseq AI is a software platform for automated in silico specificity analysis of PCR and next-generation sequencing (NGS) assays. It helps assay developers assess what their tests are expected to detect—and what they may miss—without relying on manual review of extensive alignment tables. The platform runs live BLAST searches against NCBI databases and evaluates sequence identity, mismatch position, and predicted hybridization strength.
It supports inclusivity and exclusivity checks across target strains, related species, near neighbors, and population variant sets, as well as multiplex-panel screening for primer dimers and cross-hybridization. Users can also review the potential impact of mutations, insertions, and deletions, including possible allele dropout. Each analysis produces structured results with explained scores, alignments, and versioned, timestamped records that can be exported for design and development or validation documentation.
BLASTseq AI presents example workflows for pathogen identification, germline carrier screening, and resistance-marker analysis. The company states that sequence, run, and chat data are not used to train AI models, and users can choose a data-retention window
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The BLASTseq AI name, logos and brand assets are trademarks of BLASTseq AI and its affiliates. Brandfetch isn't affiliated with or endorsed by BLASTseq AI. Their use is subject to BLASTseq AI's brand guidelines.
