FCM PASS is a software platform designed to improve rigor and reproducibility in small particle flow cytometry across instruments and laboratories. It helps researchers catalogue and manage flow cytometers, light scatter reference materials, fluorescent beads, and rainbow particles in one centralized system, reducing repetitive data entry and supporting consistent records. The software also provides tools to optimize fluorescence and light scatter detector performance, calibrate data into standardized units, and analyze flow cytometry files. Its reporting features generate transparent, shareable reports with detailed metadata, including reports aligned with MIFlowCyt and MIFlowCyt-EV guidelines.

FCM PASS supports instruments from manufacturers including Agilent, Beckman Coulter, Cytek Biosciences, nanofcm, Sony Biosciences, and Thermo Fisher Scientific. It is available for Windows and Apple Silicon Macs. Qualifying academic institutions can access free licenses, while paid commercial tiers are offered. The platform has been used by more than 600 researchers in 29 countries and featured in peer-reviewed publications, including work in Cytometry Part A, Cell Reports Methods, Nano Letters, and the Journal of Extracellular Vesicles.