Nadace Jihočeské cyklostezky (South Bohemian Cycling Routes Foundation) is a nongovernmental, nonprofit organization established in 2004 to coordinate and support cycling across the South Bohemian Region of the Czech Republic. Founded by the region, tourism organizations, a transport company, and local municipalities, it works with public bodies and other partners to develop cycling and cycle tourism.

The foundation supports public cycling events through grant programs, helping encourage healthy lifestyles, promote bicycle travel, and introduce visitors and residents to the region. It also contributes to regional planning through a cycling and transport strategy that guides the development of routes and cycling infrastructure.

A key part of its work is auditing cycling routes and maintaining cycling signs. Trained auditors check route conditions, signage, and related facilities, with findings recorded in a shared database. The foundation’s South Bohemian Cycling Map Portal provides tools for collecting audit data, proposing routes, and sharing information with partner organizations and public authorities.