It supports both human and mouse samples and offers workflows for selecting all uploaded TCRs, matching a list of target TCRs, choosing the most frequent sequences, or sampling sequences randomly. Users can also include positive controls from VDJdb, create replicate TCRs, and generate synonymous CDR3 variants with configurable minimum Hamming distance and randomization settings.

The software provides options to mutagenise both CDR3 chains or selected chain pairs, and to arrange variants as individual wells or pools. Users can select α/β or γ/δ constant-region configurations, including human or mouse choices and several α/β cassette designs. Additional controls include plate formats, plate sets, T-cell subset inclusion or exclusion, TCR naming suffixes, and maximum oligo length. Advanced settings include nested demultiplexing primer options. The site also provides example input files, lab protocols, primer sequences, feature annotations, and information about funders. The interface identifies the software as TCRAFT version 1 (published), version 2f4c932.