The Nucleic Acid Knowledgebase (NAKB) is an online portal for exploring three-dimensional structural information about nucleic acids. It succeeds the Nucleic Acid Database and brings together experimentally determined structures containing nucleic acids from the NDB and Protein Data Bank. The resource covers structures produced using major experimental methods, including X-ray crystallography, electron microscopy, and nuclear magnetic resonance.

NAKB offers tools and information for researchers, educators, and others interested in nucleic acid structures. Users can search and explore the collection, review annotations for nucleic acids, proteins, and ligands, and access reports, statistics, visualizations, and an atlas. The site also provides links to external resources that annotate and analyze nucleic acid structures and their complexes.

Additional educational and reference materials include introductions to nucleic acids, a base-pair catalog, molecule-of-the-month features, a modified nucleotide browser, and resources on standards, valence, and geometry. NAKB is supported by the National Institutes of Health’s National Institute of General Medical Sciences and serves as a community resource for structural information and analysis.